Grossmont College Chemistry Problem Set Worksheet

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  • The standard state Gibb’s free energy (ΔG°’) for synthesis of a particular acyl-CoA from its fatty acid and free Coenzyme A (CoASH) is +48.0 kJ/mol. Using this value and the information contained in Table 14-3 in your textbook, calculate the ΔG°’ value for each of the following reactions:

fatty acid + CoASH + ATP ⇌ acyl-CoA + ADP + Pi

fatty acid + CoASH + ATP ⇌ acyl-CoA + AMP + 2 Pi

Now write the equation and calculate the ΔG°’ value for the reaction as it would occur if catalyzed by the enzymes acyl-CoA synthetase and inorganic pyrophosphatase. What drives this reaction to occur spontaneously in the cytoplasm of cells?

  • Camels can wander around in the desert for long periods of time without drinking water. One reason for this is their hump that is full of fatty tissue. Imagine a camel that contains 30 pounds of triacylglycerols in its hump. How much water could be obtained by complete beta-oxidation of the fatty acids contained in this mass of triacylglycerol. Assume that all the fatty acids in your camel are the saturated 16-carbon palmitate.
  • Write out the sequence of reactions for the conversion of myristoyl-CoA to lauroyl-CoA and acetyl-CoA. Include the names of the enzymes and any necessary cofactors.
  • If malonyl-CoA is synthesized from acetyl-CoA and 14CO2, where will the 14C end appear in palmitic acid?
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